Inmunología de las Mucosas y Alergia

Integrantes

Investigadores Principales:
- Eduardo Arranz
- David Bernardo
Investigadores Doctores:
- José Antonio Garrote
- Celia Escudero
- Sara Cuesta
- Laura Romero Castillo
Investigadores Predoctorales:
- Aida Fiz
- Elisa Arribas
- Ángel de Prado
- Carolina González
- Alejandro del Hierro
- Marina Betania Pérez

Breve descripción del grupo

Our research group is devoted to the study of human intestinal mucosal immunology, both in resting conditions as well as under inflammatory processes like coeliac disease and inflammatory bowel disease. To that end, we have established a close interaction with several gastrointestinal units which provide us with biological material (blood and intestinal tissues) from patients and controls. We pay particular attention to both the intraepithelial lymphocytes, as well as to the study of the ontogeny, phenotype and function of human mucosal dendritic cell and macrophage subsets and their circulating precursors in health and under inflammatory conditions. To that end, we have several models of mucosal immunology with human primary samples and access to a top-of-the-art spectral cytometer.

Recently, we have also expanded our interest to study of COVID-19 immune response. In particular, we are interested in the development of novel biomarkers to predict disease outcome, assess the evolution of the immune response, characterize the similarities and differences of the cellular and humoral immune memory in naturally (infected) and artificially (vaccinated) individuals and to unravel the basis underlaying the long-COVID syndrome.

Last, but not least, David Bernardo is also a member of the Executive Committee of the CSIC's Global Health Thematic Interdisciplinary Platform (PTI Global Health, https://pti-saludglobal-covid19.corp.csic.es) which agglutinates over than 200 research groups to fight Global Health problems as this one. Indeed, the PI is also the coordinator (together with Dr. María Montoya) of the WP5: IMMUNE where they supervise the work of 25 independent research groups with a budget over 5 million euros.

Hitos más relevantes

E Arribas-Rodríguez, A De Prado, B de Andrés, B Velayos, J Barrio, A Romero, FJ García-Alonso, A Martín-Muñoz, JA Garrote, E Arranz, L Fernández-Salazar, D Bernardo (2025). Tofacitinib downregulates JAK1 and JAK3 on human intestinal monocytes and macrophages without affecting dendritic cells phenotype or function. Journal of Translational Autoimmunity. https://doi.org/10.1016/j.jtauto.2025.100271
A De Prado, P Cal-Sabater, A Fiz-López, S Izquierdo, D Corrales, F Pérez-Cózar, J H-Vázquez, E Arribas-Rodríguez, C Perez-Segurado, Á Martín Muñoz, JA. Garrote, E Arranz, C Marañón, S Cuesta-Sancho, L Fernández-Salazar, D Bernardo (2024). Complex immune network and regional consistency in the human gastric mucosa revealed by high-resolution spectral cytometry. Scientific Reports volume 14, 28685
J Hernandez-Vazquez, P Cal-Sabater, E Arribas-Rodríguez, A Fiz-López, C Perez-Segurado, A Martín-Muñoz, A De Prado, I de la Fuente Graciani, S Pérez González, S Gutiérrez, P Tellería, C Novoa, S Rojo Rello, A Garcia-Blesa, R Sedano, AM Martínez García, S Garcinuño Pérez, M Domínguez-Gil, C Hernán García, MM Guerra, E Muñoz-Sánchez, C Barragan-Pérez, S Diez Morales, O Casazza Donnarumma, D Ramos Pollo, N Santamarta Solla, PM Álvarez Manzanares, S Bravo, C García Alonso, A Tesedo Nieto, EC López Moreno, ME Cabrera Sanz, S Borge Olmedo, M de Paula Ortiz, A Castellanos Asenjo, J Gay Alonso, JA Garrote, E Arranz, JM Eiros, F Rescalvo Santiago, C Quevedo Villegas, E Tamayo, A Orduña, C Dueñas, MJ Peñarrubia, S Cuesta-Sancho, M Montoya, D Bernardo (2024). Unbiased spectral cytometry immunome characterization predicts COVID-19 mRNA vaccine failure in older adults and patients with lymphoid malignancies. Frontiers Immunol Volumen 15. doi: 10.3389/fimmu.2024.1405217
A Fiz-López, Á De Prado, E Arribas-Rodríguez, FJ García-Alonso, S Izquierdo, A Martín-Muñoz, JA Garrote, E Arranz, J Barrio, L Fernández-Salazar, D Bernardo (2024). Biological variability of human intraepithelial lymphocytes throughout the human gastrointestinal tract in health and coeliac disease. Eur J Clin Invest. doi: 10.1111/eci.14304. Online ahead of print.PMID: 39210517
E Arribas-Rodríguez, L Fernández-Salazar, B de Andrés, E Arranz, JA Garrote, D Bernardo. Study and isolation of human intestinal dendritic cell and macrophage subsets. Methods in Cell Biology. 2023. In press
G Barturen, E Carnero-Montoro, M Martínez-Bueno, S Rojo-Rello, B Sobrino, O Porras, C Alcántara-Domínguez, D Bernardo, M Alarcon-Riquelme. Whole-Blood DNA Methylation Analysis Reveals Respiratory Environmental Traits Involved in COVID-19 Severity Following SARS-CoV-2 Infection. Nature Communications. 2022. NCOMMS-21-29579C]
COVID-19 Host Genetics Initiative. Mapping the human genetic architecture of COVID-19. Nature. 2021 Dec;600(7889):472-477. doi: 10.1038/s41586-021-03767-x. * David Bernardo is a member of the COVID-19 Host Genetics Initiative
A Cossarizza, HD Chang, A Radbruch, S Abrignani, R Addo, M Akdis, I Andrä, F Andreata, F Annunziato, E Arranz, P Bacher, S Bar, V Barnaba, J Barros-Martins, D Baumjohann, CG Beccaria, D Bernardo, DA Boardman, J Borger, C Böttcher, L Brockmann, M Burns, DH Busch, G Cameron, I Cammarata, A Cassotta, Y Chang, FG Chirdo, E Christakou, L ˇCiˇcin-Šain, L Cook, AJ Corbett, R Cornelis, L Cosmi, MS Davey, S De Biasi, G De Simone, G del Zotto, M Delacher, F Di Rosa, J Di Santo, A Diefenbach, J Dong, T Dörner, RJ Dress, CA Dutertre, SBG Eckle, P Eede, M Evrard, CS Falk, M Feuerer, S Fillatreau, A Fiz-Lopez, M Follo, GA Foulds, J Fröbel, N Gagliani, G Galletti, A Gangaev, No Garbi, JA Garrote, J Geginat, NA Gherardin, L Gibellini, F Ginhoux, DI Godfrey, P Gruarin, C Haftmann, L Hansmann, CM Harpur, AC Hayday, G Heine, DC Hernández, M Herrmann, O Hoelsken, Q Huang, S Huber, JE Huber, J Huehn, M Hundemer, WYK Hwang, M Iannacone, SM Ivison, HM Jäck, PK Jani, B Keller, N Kessler, S Ketelaars, L Knop, J Knopf, HF Koay, K Kobow, K Kriegsmann, H Kristyanto, A Krueger, JF Kuehne, H Kunze-Schumacher, P Kvistborg, I Kwok, D Latorre, D Lenz, MK Levings, AC Lino, F Liotta, HM Long, E Lugli, KN MacDonald, L Maggi, MK Maini, F Mair, C Manta, RA Manz, MF Mashreghi, A Mazzoni, J McCluskey, HE Mei, F Melchers, S Melzer, D Mielenz, L Monin, L Moretta, G Multhoff, LE Muñoz, M Muñoz-Ruiz, F Muscate, A Natalini, K Neumann, LG Ng, A Niedobitek, J Niemz, LN Almeida, S Notarbartolo, L Ostendorf, LJ Pallett, AA Patel, GI Percin, G Peruzzi, M Pinti, AG Pockley, K Pracht, I Prinz, I Pujol-Autonell, N Pulvirenti, L Quatrini, KM Quinn, H Radbruch, H Rhys, MB Rodrigo, C Romagnani, C Saggau, S Sakaguchi, F Sallusto, L Sanderink, I Sandrock, C Schauer, A Scheffold, HU Scherer, M Schiemann, FA Schildberg, K Schober, J Schoen, W Schuh, T Schüler, AR Schulz, S Schulz, J Schulze, S Simonetti, J Singh, KM Sitnik, R Stark, S Starossom, C Stehle, F Szelinski, L Tan, A Tarnok, J Tornack, TIM Tree, JJP van Beek, W van de Veen, K van Gisbergen, C Vasco, NA Verheyden, A von Borstel, KA Ward-Hartstonge, K Warnatz, C Waskow, A Wiedemann, A Wilharm, J Wing, O Wirz, J Wittner, JHM Yang, J Yang. Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition). Eur J Immunol, 2021 Dec;51(12):2708-3145. doi: 10.1002/eji.202170126.
A Tamayo-Velasco, MJ Peñarrubia Ponce, FJ Álvarez, H Gonzalo-Benito, I de la Fuente, S Pérez-González, L Rico, MT Jiménez García, A Sánchez Rodríguez, M Hijas Villaizan, M Martín-Fernández, C Dueñas, E Gómez-Sánchez, M Heredia-Rodríguez, O Gorgojo-Galindo, I Fernández, L del Río, I Carnicero-Frutos, MF Muñoz-Moreno, E Tamayo*, E Martínez-Paz*, D Bernardo*. Can the cytokine profile according to ABO blood groups be related to worse outcome in COVID-19 patients? Yes, they can. Frontiers in Immunology, 2021, https://doi.org/10.3389/fimmu.2021.726283
S Fernández-Tomé, P Indiano-Romacho, I Mora-Gutiérrez, L Pérez-Rodríguez, L Ortega Moreno, AC Marin, M Baldán-Martín, JA Moreno-Monteagudo, C Santander, M Chaparro, B Hernández-Ledesma, JP Gisbert, D Bernardo. Lunasin peptide is a modulator of the immune response in the human gastrointestinal tract. Mol Nutr Food Res. 2021 Jun;65(12):e2001034.
L Ortega-Moreno, S Fernández-Tomé, M Chaparro, AC Marin, I Mora-Gutiérrez, C Santander, M Baldan-Martin, JP Gisbert, D. Bernardo. Profiling of Human Circulating Dendritic Cells and Monocyte Subsets Discriminates Between Type and Mucosal Status in Patients With Inflammatory Bowel Disease. Inflamm Bowel Dis. 2021 Jan 19;27(2):268-274. doi: 10.1093/ibd/izaa151.
C Escudero-Hernández, A Martín, R de Pedro Andrés, L Fernández-Salazar, JA Garrote, D Bernardo, E Arranz. Circulating Dendritic Cells From Celiac Disease Patients Display a Gut-Homing Profile and Are Differentially Modulated by Different Gliadin-Derived Peptides. Mol Nutr Food Res. 2020; 64(6):e1900989. doi: 10.1002/mnfr.201900989.
S. Fernández-Tomé, B. Hernández-Ledesma, M. Chaparro, P. Indiano-Romacho, J.P. Gisbert*, D. Bernardo*. Role of food proteins and bioactive peptides in inflammatory bowel disease. Trends in Food Science and Technology. 2019. 88:194-206.*equal contribution
M. Martínez-López, S. Iborra, R. Conde-Garrosa, A. Mastrangelo, C. Danne, E.R. Mann, D.M. Reid, V. Gaboriau-Routhiau, M. Chaparro, M.P. Lorenzo, L. Minnerup, P. Saz-Leal, E. Slack, B. Kemp, J.P. Gisbert, A. Dzionek, M.J. Robinson, F.J. Rupérez, N. Cerf-Bensussan, G.D. Brown, D. Bernardo, S. LeibundGut-Landmann, D. Sancho. Microbiota sensing by Mincle-Syk axis in dendritic cells regulates IL-17 and IL-22 and promotes intestinal immune barrier. Immunity. 2019. 50. 1-16.

Consulta en Pubmed todas las publicaciones

Proyectos destacados

Título del proyecto: Descifrando la capacidad inmunoreguladora de vista en el intestino humano en la enfermedad inflamatoria intestinal (PID2023-148270OB-I00).

Duración: 2024 – 2027.

Entidad financiadora: Ministerio de Ciencia, Innovación y Universidades, Agencia Estatal de Investigación y FEDER, UE.

Presupuesto: 125.000 €.

Investigador Principal: David Bernardo Ordiz.

Proyecto PID2023-148270OB-I00 financiado por:

2022-2026 Proyecto de Infraestructura en Red de Castilla y Leon (INFRARED) (IR2021-UVA04) (484.237 €).

"Citómetro tipo sorter espectral de 3 láseres y 38 detectores".

IP: David Bernardo.

2020-2024 Proyecto de Infraestructura en Red de Castilla y Leon (INFRARED) (IR2020-1-UVA01) (490.520 €).

"Citómetro espectral equipado con 5 láseres (355 nm, 405 nm, 488 nm, 561 nm, 638 nm) y con 67 detectores".

IP: David Bernardo.

2020-2023 Proyecto de investigación financiado por el Ministerio de Ciencia e Innovación (PID2019-104218RB-I00) (139.150 €).

"Migración y diferenciación de las subpoblaciones de células dendríticas y monocitos en la mucosa intestinal de pacientes con enfermedad inflamatoria intestinal".

IP: David Bernardo.

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